000 | 01248am a2200193a 44500 | ||
---|---|---|---|
092 |
_a04 _b |
||
260 | _c1996 | ||
300 | _a215-223 | ||
650 | _aPecten maximus | ||
650 | _avieira | ||
650 | _aBacteria | ||
650 | _aNutrition | ||
650 | _aGreat Scallop | ||
650 | _alarvae | ||
650 | _aTracer technique | ||
520 | _aBacterial nutrition of great scallop, Pecten moximus, larvae was investigated using the radioactive tracer technique. The bacterial labelling was studied initially to obtain a high and stable specific radioactivity (14C) of bacterial cells. A higher bacterial specific 14C activity was obtained when the tracer (amino acid) was introduced in the culture medium at the beginning of the exponential growth phase. After a 12 h labelling period in a rich nutritive medium, the bacteria were depurated in seawater for 5 h (chase) to prevent further 14C excretion and then added to larval rearing vessels. The larval labelling was followed for 12 h and then larvae were placed in new vessels without radioactive bacteria. The depuration of larvae was followed for 3 days. Data obtained on ingestion and assimilation efficiency show that bivalve larvae are able to ingest and digest bacteria. | ||
942 | _cBK | ||
999 |
_c514 _d514 |